Missing You......

It's already been weeks...three weeks for the taking...that I haven't heard of even a simple 'hi' nor 'hello' from my guy. The last conversation we have was when it was a 'free call', as I can still remember. The 'free call' by SMART network. All you have to do in order to avail for such call is dial a certain number and then that's it. But after that call, here comes the empytiness in me. I am sad, yes of course so sad. He never even bother to know what's happening to me. We are distant apart. Far from each other. I can say miles, miles apart. But long distance affair is not that bad right? I don't have any idea neither a single clue on what's happening to him out there. It makes me lose hope that this relationship would no longer fluorish to the way we want it to be. Relationship without communication is useless. Somehow, I believe in long distance relationship that would last. But how would it last if there's no communication in both side. I really do missed him a lot and how I wish I could make him stay with me. But is it just for a simple wish? Everytime my phone beeps and I found out that it's not from him, I feel so empty inside. I wanna cry. I can no longer bear the emptiness and longingness for him.
Oh bother! Where is he right now? What is he doing? Who is with him? I really don't know the answers. I'm so lonely and in doubt. Can he still remember me? Can he make it up to me? What if for the whole month I don't even get a single message? What would I do?
He's all I ever wanted. I don't know if I can take it if I found out something. I just hope that I am wrong with what I am thinking. Without him is like a thorn in a bush. No one dares to touch it, afraid to get a wound and bleed.
Where are you now? Where?...

How Lucky Could I be?..

Do you ever find yourself being too lucky each day? Can you be called a 'lucky one' if you know, you want to have it all but you just simply can't have it? Life is a matter of give and take, I guess. But as for a girl like me, I don't know if I'm suited enough to be a part of those whom being called 'lucky one'...

How lucky could I be to have...

• a FAMILY and yet broken;
• a GUY whom I longed to be mine but never shows he cares;
• a WORK but with very little amount I get and always not on time;
• FRIENDS to accompany but don't know who are real;
• PLANS for the future but turned out to be only a plan and never meant for real;
• and CHANCES for survival but never knows the real mission in life.

Is this the real thought of being a 'lucky one'. Well, maybe yes and maybe no. But as time goes by this is what I discover. Real happiness isn't yet here for me but somehow I'm longing for it. Only time can tell if I could be a part of those lucky ones. Somehow, let me find that single fortune in my life that would ever make my whole life complete. As for you, how do you consider yourself a lucky one?

A Special World

This is a
special world for you and me
A special kind of bond one cannot see
It enfolds us up in its cocoon
And holds us sternly in its womb.

Its fingers spread like fine spun gold
Gently nestling us to the fold
Like silken thread it grasps us fast
Bonds like this are meant to last.

And though at times a thread may break
A new one forms in its wake
To bind us closer and keep us strong
In a special world, where we belong.

ELISA: A Means of Detection

ELISA is a medical jargon that is confusing especially to those people who are not medical practitioners. What is the phrase all about? What does it signifies? ELISA is a primary tool of clinical immunology which is used as a preliminary screen for HIV detection. HIV or human immunodeficiency virus causes AIDS (Acquired Immunodeficiency Syndrome) which is a disease that makes it difficult for the body to fight off infectious diseases. HIV can be transmitted through direct contact with the blood or body fluid of someone who is infected with the virus. Thus, HIV ELISA which is also called as HIV enzyme immunoassay (EIA) is the appropriate test to determine if an individual is positive for a selected pathogen. The test is performed in a 8 cm x 12 cm plastic plate which contains an 8 x 12 matrix of 96 wells, each of which are about 1 cm high and 0.7 cm in diameter.

Because ELISA can be performed to weigh up the presence of antigen or even the presence of antibody in a sample, it has also found applications in the food industry in distinguishing potential food allergens such as peanuts, almonds, walnuts, milk, and eggs. Not only that, ELISA can be used in toxicology as a quick presumptive screen for certain classes of drugs.

Because EIA or Enzyme Immunoassay test is used to distinguish and calculate specific antigen-eliciting molecules involved in biological processes, it can be used too on most types of biological samples such as that of plasma, urine, serum and cell extracts. In the assay, a plate is coated with a most important antibody, which recognizes the antigen of the target molecule and bonds with it. The antigen-antibody complex is recognized by a minor antibody that is joined to an enzyme that catalyzes the reaction mixture, deferring a specific color. By measuring the optical density of this color, the occurrence and number of a specific molecule can be determined; the density of color is proportional to the advancement of the reaction or disease being tested.

If an ELISA test is used for drug screening at workplace, a cut-off concentration, 50 ng/mL, is ascertained, and a sample will be prepared which contains the average application of analyte. Unknowns that generate a signal that is stronger than the known sample are "positive". Those that generate weaker signal are "negative”. Also, ELISA test is done for increasing community consciousness of any disease, promoting recognition of its warning signs to seek treatment and further more to provide information with regards to the accessibility of appropriate professional services locally and farther afield.

I AM FREE.!.

The time has come...
I finally made up my mind...
I have moved on...
the agony & pain is gone...
I'm free!

yes, I AM FREE!!!

Free as the clouds above the sky...
free as a bird soaring up high...
free as the waves on the ocean...
free as the sounds on the hills...
and free as the rain pouring from the sky...
yes, this is it...
I did surpass it...I did overcome it......